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HAYWARD, Calif.--(BUSINESS WIRE)--Aug. 11, 2003--Guava Pca 96 System User's Manual Instructions
Company Additionally Releases 96-Well Versions of Its MultiCaspase, TUNEL and Cell Cycle Assays |
Screening individual cells for green fluorescent proteins is now easier and quicker than ever for drug discovery researchers with the new Guava PCA-96 AFP (Auto-Fluorescing Protein) system, announced Guava Technologies, Inc. The compact Guava PCA-96 AFP allows researchers to determine the relative levels of fluorescing proteins produced by individual cells in a culture and to screen 96 such cultures in a single instrument run. The new Guava PCA-96 AFP system is the only blue-laser, micro-volume cell analysis system capable of screening single cells for GFP expression in an automated 96-well plate format at the research benchtop.
Guava Technologies is introducing the Guava PCA-96 AFP system to the scientific community this week at the 'Drug Discovery Technology' meeting and exhibition held August 10-15 in Boston, Mass.
Guava additionally announced that its popular Guava PCA assays MultiCaspase, TUNEL, and Cell Cycle are now available in the 96-well microplate format for use with the Guava PCA-96 system. Each of these new 96-well plate tests, as well as Guava's previously available ViaCount, Express and Nexin microplate assays, allows users to grow, treat, stain, incubate and acquire non-adherent (and some adherent) cells directly from the same microplate. The result is greatly streamlined sample preparation and minimized cell handling.
'These new 96-well format products reflect Guava's ongoing commitment to providing life science researchers and biopharmaceutical manufacturers with easy-to-use, benchtop, single-cell analysis capabilities that significantly accelerate the pace of their efforts in both the lab and the cell-processing facility,' said Kim Mulcahy, director of product marketing.
GFP Screening at the Benchtop
May 29, 2007 Guava Express is one of several modules contained within the Guava Cytosoft program suite that comes with Guava’s personal cell analysis systems. My experiences are with the version of software bundled with the Guava PCA 96 System. Through our partnership with GoIndustry DoveBid, we have greatly reduced the amount of time spent on commercial work - from price negotiations, contract checking and signing, invoicing to pickup and delivery of equipment. May 15, 2007 Allogenically stimulated effector PBMCs were mixed with target cells from the same donor at various ratios and run on a Guava PCA-96 System. Figure 3 shows that the Guava.
Naturally fluorescing proteins provide cell biologists with the means to track the expression, production and movement of proteins within living cells. While GFP is the most commonly used fluorescent variant, proteins that fluoresce in the yellow, red and blue range are also in use. The most commonly used variant of GFP for drug discovery and other mammalian cell research is most efficiently excited by light from a blue laser. Blue lasers are key components of flow cytometers, complex instruments that researchers can use for GFP expression studies to quantify the amount of a target protein expressed within a cell, on the cell surface, or within the supernatant from a cell culture. However, use of flow cytometry in drug discovery research has been constrained due to the high instrument cost, difficulty of use, and the difficulty of scheduling experiment time on cytometers, which are maintained by specialized technicians in core facilities outside of most laboratories. Moreover, current 96-well microplate attachments for flow cytometers have been plagued by problems of significant sample carryover from well to well that greatly skew the accuracy and reliability of test results. Fluorescent plate readers can detect GFP in microplates and can easily fit on benchtops but can only provide data on whole populations of cells rather than individual cells, thus limiting assay sensitivity.
'The Guava PCA-96 AFP's fluorescence sensitivity, ease of use, and ability to analyze only microliter volumes (without sample carryover) makes the new Guava quite versatile as a research instrument and a terrific addition to the Guava product line,' said Dr. Lewis Lanier, professor of microbiology and immunology at the University of California, San Francisco. 'The Guava PCA-96 AFP system is really useful for analyzing transformed cells that express GFP and detecting cells stained with both FITC-conjugated and PE-conjugated antibodies.'
Mar 16, 2017 The Guava Easycyte also offers integrated, turnkey operations. The Guava Easycyte is the latest addition to Guava's range of Personal Cell Analysis systems, which includes the Guava PCA and the Guava PCA-96, and is designed to save precious cells and reagents reducing potentially hazardous biological waste. PCA-6782 User Manual iv Product Warranty (2 years) Advantech warrants to you, the original purchaser, that each of its products will be free from defects in materials and workmanship for two years from the date of pur.
Like other Guava PCA systems, the Guava PCA-96 AFP is compact, affordable and easy to use, requiring only microvolumes of cells and reagents and less than a day's training.
Blitzkrieg manual download. Cellular High Throughput Screening with Guava PCA-96 Highlighted
The power of Guava's previously introduced Guava PCA-96 system for the rapid and objective analysis of single cells in the 96-well plate format was highlighted at the Drug Discovery Technology meeting in a poster abstract and a presentation by Guava Technologies' scientists. Guava offers a growing variety of biological applications for use with the Guava PCA-96 system. These include Guava's leading ViaCount assay for cell counting and viability studies; Express for protein and antigen expression; Nexin for early apoptosis detection; and new PCA-96 format assays MultiCaspase and TUNEL, for mid- and late stage apoptosis, and a Cell Cycle assay.
Guava Technologies scientists presented research demonstrating the use of the Guava PCA-96 to rapidly, precisely and objectively perform single cell analysis using a variety of applications. The researchers measured not only cell concentration and overall viability, but were able to achieve a comprehensive picture of cell growth, death and response to a variety of compounds, including cell cycle inhibitors and apoptosis-inducing agents.
'The Guava PCA-96 is useful not only in drug discovery and development, but also in downstream areas of the drug development process, such as pilot production and manufacturing where batch preparations of drug compounds must be tested for potency and stability,' said Dianne Fishwild, Ph.D., Guava Technologies director, applications development. 'Moreover, researchers can quickly and effectively obtain meaningful results using the Guava PCA-96 system with only small volumes of precious reagents and small numbers of cells. As a result, potential cytotoxic and cytostatic drugs can be quickly and effectively evaluated in cell-based assays, right at the laboratory benchtop.'
About Guava Technologies
Guava Pca 96 System User's Manual Form
Based in Hayward, Calif., Guava Technologies is a biotechnology company that develops, manufactures and markets patented, integrated cellular analysis systems for the life science researcher's benchtop. The company is revolutionizing the way that cell culture monitoring and cell screening assays are performed, by providing highly miniaturized microliter scale systems that make common assays in cell-based analysis accessible at the benchtop. Guava's products have broad applications to life sciences research, drug discovery and biopharmaceutical production today, but also offer potential for use in clinical testing and blood processing, as well as other areas where single cell analysis is important. More information about the company and its products is available at www.guavatechnologies.com.
Guava, Guava Technologies and ViaCount are registered trademarks of Guava Technologies, Inc. PCA, Nexin, and MultiCaspase are trademarks of Guava Technologies, Inc.